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dnmA [2020-09-08 17:07:07]
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dnmA [2020-09-08 17:07:07]

DNA methyltransferase
locus
BSU_06760
pI
5.67
mw
100.96 kDa
protein length
879 aa Sequence Blast
gene length
2640 bp Sequence Blast
function
genome-wide N6-methyladenosine modifications at non-palindromic recognition sites
product
DNA methyltransferase
essential
no
synonyms
yeeA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.9|Other regulators]

    • Gene

    Coordinates
    740,288 → 742,927

    The protein

    Catalyzed reaction/ biological activity

  • formation of m6A DNA modifications at non-palindromic sites [pubmed|32324221]

    • Structure

  • [PDB|5HR4] (from Methylophilus methylotrophus, 38% identity) [pubmed|27082731]

    • Expression and Regulation

    Operons

    genes
    [gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A959 (yeeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/959 NBRP B. subtilis, Japan]
  • BKE06760 (Δ[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA, downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG
  • BKK06760 (Δ[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA, downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG

    • References

    Research papers

  • 27082731,32324221